Ho theha mekhoa ea tlhahlobo ea motsoako (li)
Re tsepamisitse maikutlo haholo taolong ea boleng, mosebetsi oa rona o pharaletseng oa lipatlisiso le nts'etsopele o fihlile sehlohlolong ka ho theha mekhoa ea tlhahlobo ea maemo a holimo, e tiisang liphetho tse nepahetseng le tse tšepahalang.
Bohareng ba linyehelo tsa rona ke boitlamo ba rona ba ho fana ka mekhoa e netefalitsoeng ea tlhahlobo e lumellang bareki ba rona ho netefatsa boteng ba lintho tse sebetsang ka nepo. Re utloisisa bohlokoa ba ho ba le mekhoa ea tlhahlobo e matla le e hlokang kelello ho fumana matla le bohloeki ba metsoako e sebetsang. Ka hona, re qapile letoto la mekhoa ea tlhahlobo e sa nolofatseng feela ts'ebetso ea tlhahlobo, empa hape e fana ka motheo o tiileng oa mehato ea taolo ea boleng.
E 'ngoe ea matla a bohlokoa a mekhoa ea rona ea tlhahlobo ke boemo ba ts'epo boo ba bo kenyang ho bareki. Ka lisebelisoa tsa rona tse tšepahalang, ba ka kholiseha hore ba na le mokhoa o hlokang kelello oa ho laola boleng ba lihlahisoa tsa bona. Sena le sona se ba thusa ho hlaolela ts'epo le ts'epo 'marakeng, ka hona ba ntlafatsa seriti sa bona le ho netefatsa khotsofalo ea bareki.
Lenaneo la rona le felletseng la mekhoa ea tlhahlobo le akaretsa mekhoa e mengata e fapaneng ea ho fihlela litlhoko tse fapaneng tsa liindasteri le lihlahisoa tse fapaneng. High Performance Thin Layer Chromatography (HPTLC) ke mokhoa o mong oo re o entseng ka katleho. Theknoloji e fana ka bokhoni bo phahameng ba karohano le boitsebahatso, bo lumellang boikemisetso bo nepahetseng ba metsoako e sebetsang mefuteng e mengata e fapaneng.
Ntle le moo, re sebelisa chromatography ea metsi e sebetsang hantle haholo (HPLC) mekhoeng ea rona ea tlhahlobo ho ntlafatsa ho nepahala le ho sebetsa hantle. HPLC ke mokhoa o feto-fetohang o ka arolang, oa lekanya, 'me oa tsebahatsa lintho tse sebetsang ka har'a metsoako e rarahaneng. Ka ho kenyelletsa HPLC mekhoeng ea rona ea tlhahlobo, re fa bareki ba rona litharollo tse felletseng tse akaretsang litlhoko tse ngata tsa tlhahlobo.
Leha litheknoloji tsena tse peli li theha motheo oa mokhoa oa rona oa tlhahlobo, rea utloisisa hore sehlahisoa ka seng se na le litšobotsi le litlhoko tse ikhethang. Ka hona, ho netefatsa mokhoa o entsoeng ka mokhoa o ikhethileng, re ikemiselitse ho hlophisa mokhoa oa rona ho fihlela litlhoko tse ikhethileng tsa bareki ba rona. Morero o mong le o mong o reriloe le ho etsoa ka hloko, ho nahanoa ka ho rarahana le ho rarahana ha metsoako e sebetsang e ntseng e nahanoa.
Ka kopanelo, boiteko ba rona bo pharaletseng ba lipatlisiso le nts'etsopele bo fihlile sehlohlolong sa ho theha mekhoa ea morao-rao ea tlhahlobo, ho fa bareki ba rona lisebelisoa tse tšepahalang ho laola boleng ba lihlahisoa tsa bona. Ka boitseanape ba rona ho HPTLC, HPLC le mekhoa e meng ea sejoale-joale, re netefatsa boitsebahatso bo nepahetseng, palo le netefatso ea metsoako e sebetsang mekhoeng e fapaneng. Lumela hore re tla ba molekane oa hau oa leano ho netefatsa boleng ba sehlahisoa, botumo le katleho ea 'maraka.